ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (12): 2441-2449.doi: 10.11843/j.issn.0366-6964.2016.12.014

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Effects of Starter Feeding on Rumen Papilla Genes Expression Involved in Cellular Growth and Morphology in Hu Lamb at Different Ages

LIU Ting1，LI Fa-di1* ，WANG Wei-min1，2，WANG Xiao-juan1，LI Chong1，2，LI Fei3，ZHENG Chen1，MO Fu-tao1，WANG Fang-bin1，LA Yong-fu1，LI Bao-sheng4   

  1. (1.College of Animal Science and Technology，Gansu Agricultural University，Lanzhou 730070，China；2.Engineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province，Minqin 733300，China；3.College of Pastoral Agriculture Science and Technology，Lanzhou University，Lanzhou 730020，China；4.Jinchang Zhongtian Sheep Industry Co.Ltd，Jinchang 737100，China)
  • Received:2015-12-31 Online:2016-12-23 Published:2016-12-23

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Abstract:

The objective of this study was to investigate the effects of starter age on Hu lamb genes expression involved in cellular growth in rumen papilla and rumen morphology.Two factor design (starter age and age of lambs) was adopted in this study.A total of 78 male Hu lambs with similar born body weight （(3.41±0.27) kg） were selected.Of which，six were slaughtered immediately after birth and the remaining 72 were equally divided into 2 groups，a group fed by starter diet 1 at d 7 and a group fed by starter diet 1 at d 42，and lambs from both groups were weaned at d 56 and transacted to starter diet 2 at d 60.The transition period lasted 10 d.Six lambs from each group were slaughtered at d 14，28，42，56，70 and 84，respectively.The ventral sac of the rumen was collected，rumen papilla height，width and muscular thickness were measured，and total RNA was extracted.Real-time qPCR was used to analyze the expression of genes related to rumen papilla growth.Tissue sections were prepared to observe rumen development.The results revealed that rumen papilla width of 7 d-starter group was higher (P<0.05) than that of 42 d-starter group，however，rumen muscular thickness in 7 d-starter group was lower (P<0.05) than that in 42 d-starter group.The IGFBP3 expression of rumen tissue in 7 d-starter group were lower (P<0.05) than that in 42 d-starter group.In contrast，IGFBP5 expression in 7 d-starter group were higher (P<0.05) than that in 42 d-starter group.In 7 d-starter group，rumen papilla height and width were positively correlated with expression of TGFβ1 and IGFBP3 (R=0.507，P<0.001；R=0.444，P<0.001；R=0.465，P<0.001；R=0.299，P=0.011)，while were negatively correlated with expression of IGFBP6 (R=－0.443，P<0.001；R=－0.477，P<0.001).In 42 d-starter group，rumen papilla height was positively correlated with expression of IGFBP5 (R=0.227，P=0.018)，additionally，rumen papilla width were positively correlated with expression of IGFBP3 and IGFBP5 (R=0.338，P=0.005；R=0.293，P=0.002).In conclusion，this study result indicate that starter feeding for lambs at day 7 can improve rumen morphology，and the expression levels of genes involved in cellular growth in ruminal epithelium can regulate rumen development.

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